![]() In: Plant genome editing with CRISPR systems. Liu J, Gunapati S, Mihelich NT, Stec AO, Michno J-M, Stupar RM (2019) Genome editing in soybean with CRISPR/Cas9. Lakhssassi N, Zhou Z, Liu S, Piya S, Cullen MA, El Baze A, Knizia D, Patil GB, Badad O, Embaby MG (2020) Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules. Patil GB, Lakhssassi N, Wan J, Song L, Zhou Z, Klepadlo M, Vuong TD, Stec AO, Kahil SS, Colantonio V, Valliyodan B, Rice JH, Piya S, Hewezi T, Stupar RM, Meksem K, Nguyen HT (2019) Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode. Plant Genome 2021:e20109ĭeshmukh R, Rana N, Liu Y, Zeng S, Agarwal G, Sonah H, Varshney R, Joshi T, Patil GB, Nguyen HT (2021) Soybean transporter database: a comprehensive database for identification and exploration of natural variants in soybean transporter genes. Sci Data 8(1):1–9īayer PE, Valliyodan B, Hu H, Marsh JI, Yuan Y, Vuong TD, Patil G, Song Q, Batley J, Varshney RK (2021) Sequencing the USDA core soybean collection reveals gene loss during domestication and breeding. Valliyodan B, Brown AV, Wang J, Patil G, Liu Y, Otyama PI, Nelson RT, Vuong T, Song Q, Musket TA (2021) Genetic variation among 481 diverse soybean accessions, inferred from genomic re-sequencing. Patil G, Mian R, Vuong T, Pantalone V, Song Q, Chen P, Shannon GJ, Carter TC, Nguyen HT (2017) Molecular mapping and genomics of soybean seed protein: a review and perspective for the future. In: Advances in plant breeding strategies: legumes. Plant Cell 28(7):1510–1520Īnderson EJ, Ali ML, Beavis WD, Chen P, Clemente TE, Diers BW, Graef GL, Grassini P, Hyten DL, McHale LK (2019) Soybean breeding: history, improvement, production and future opportunities. ![]() Curr Opin Biotechnol 49:35–41Īltpeter F, Springer NM, Bartley LE, Blechl AE, Brutnell TP, Citovsky V, Conrad LJ, Gelvin SB, Jackson DP, Kausch AP (2016) Advancing crop transformation in the era of genome editing. Van Eck J (2018) Genome editing and plant transformation of solanaceous food crops. Graham N, Patil GB, Bubeck DM, Dobert RC, Glenn KC, Gutsche AT, Kumar S, Lindbo JA, Maas L, May GD (2020) Plant genome editing and the relevance of off-target changes. Vats S, Kumawat S, Kumar V, Patil GB, Joshi T, Sonah H, Sharma TR, Deshmukh R (2019) Genome editing in plants: exploration of technological advancements and challenges. We also describe a method for gene editing in soybean protoplasts using single guide RNA molecules. This chapter provides improved working protocols for isolating and transforming protoplast from immature soybean seeds with 44% of transfection efficiency validated by the green fluorescent protein reporter. There is a great deal of interest in the plant community to develop these precise edits, as they may expand the potential for developing value-added traits which may be difficult to achieve by other gene-editing applications and/or traditional breeding alone. It also facilitates the delivery of homology-directed repair templates (donor molecules) into plant cells, enabling precise DNA edits in the genome. This system allows the direct delivery of DNA, RNA, or proteins into plant cells and provides a high-throughput system to validate gene-editing reagents. Protoplast engineering has become an important tool for basic plant molecular biology research and developing genome-edited crops. Protoplast is a versatile system for conducting cell-based assays, analyzing diverse signaling pathways, studying functions of cellular machineries, and functional genomics screening.
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