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![]() In: Plant genome editing with CRISPR systems. Liu J, Gunapati S, Mihelich NT, Stec AO, Michno J-M, Stupar RM (2019) Genome editing in soybean with CRISPR/Cas9. Lakhssassi N, Zhou Z, Liu S, Piya S, Cullen MA, El Baze A, Knizia D, Patil GB, Badad O, Embaby MG (2020) Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules. Patil GB, Lakhssassi N, Wan J, Song L, Zhou Z, Klepadlo M, Vuong TD, Stec AO, Kahil SS, Colantonio V, Valliyodan B, Rice JH, Piya S, Hewezi T, Stupar RM, Meksem K, Nguyen HT (2019) Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode. Plant Genome 2021:e20109ĭeshmukh R, Rana N, Liu Y, Zeng S, Agarwal G, Sonah H, Varshney R, Joshi T, Patil GB, Nguyen HT (2021) Soybean transporter database: a comprehensive database for identification and exploration of natural variants in soybean transporter genes. Sci Data 8(1):1–9īayer PE, Valliyodan B, Hu H, Marsh JI, Yuan Y, Vuong TD, Patil G, Song Q, Batley J, Varshney RK (2021) Sequencing the USDA core soybean collection reveals gene loss during domestication and breeding. Valliyodan B, Brown AV, Wang J, Patil G, Liu Y, Otyama PI, Nelson RT, Vuong T, Song Q, Musket TA (2021) Genetic variation among 481 diverse soybean accessions, inferred from genomic re-sequencing. Patil G, Mian R, Vuong T, Pantalone V, Song Q, Chen P, Shannon GJ, Carter TC, Nguyen HT (2017) Molecular mapping and genomics of soybean seed protein: a review and perspective for the future. In: Advances in plant breeding strategies: legumes. Plant Cell 28(7):1510–1520Īnderson EJ, Ali ML, Beavis WD, Chen P, Clemente TE, Diers BW, Graef GL, Grassini P, Hyten DL, McHale LK (2019) Soybean breeding: history, improvement, production and future opportunities. ![]() Curr Opin Biotechnol 49:35–41Īltpeter F, Springer NM, Bartley LE, Blechl AE, Brutnell TP, Citovsky V, Conrad LJ, Gelvin SB, Jackson DP, Kausch AP (2016) Advancing crop transformation in the era of genome editing. Van Eck J (2018) Genome editing and plant transformation of solanaceous food crops. Graham N, Patil GB, Bubeck DM, Dobert RC, Glenn KC, Gutsche AT, Kumar S, Lindbo JA, Maas L, May GD (2020) Plant genome editing and the relevance of off-target changes. Vats S, Kumawat S, Kumar V, Patil GB, Joshi T, Sonah H, Sharma TR, Deshmukh R (2019) Genome editing in plants: exploration of technological advancements and challenges. We also describe a method for gene editing in soybean protoplasts using single guide RNA molecules. This chapter provides improved working protocols for isolating and transforming protoplast from immature soybean seeds with 44% of transfection efficiency validated by the green fluorescent protein reporter. There is a great deal of interest in the plant community to develop these precise edits, as they may expand the potential for developing value-added traits which may be difficult to achieve by other gene-editing applications and/or traditional breeding alone. It also facilitates the delivery of homology-directed repair templates (donor molecules) into plant cells, enabling precise DNA edits in the genome. This system allows the direct delivery of DNA, RNA, or proteins into plant cells and provides a high-throughput system to validate gene-editing reagents. Protoplast engineering has become an important tool for basic plant molecular biology research and developing genome-edited crops. Protoplast is a versatile system for conducting cell-based assays, analyzing diverse signaling pathways, studying functions of cellular machineries, and functional genomics screening. ![]() This course comes with a 365-day guarantee. ![]() It also includes several bonus contents, including The Ad Playbook Walkthrough, Youtube Ad Campaign Setup, Facebook Ad Campaign Setup, Freelance Editor Email Scripts, and Study These Proven Ads. * Creating Entertaining, Information, and Effective Ads Some are as short as 3 to 16 minutes, while others are as long as an hour. These are the Digital Technology Training, The $1,000,000 Product Launch, and the Marketing Template Vault. There are three bonus training available. The last two weeks are all about the Roadmap to a 7-Figure Future and Successful Mindset Secrets. Continue to Week 9 to learn more about Lucrative Marketing Funnels, and Week 10 to learn about Product Launch and Sales Mastery. 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The creation of the internet and its popularity over the years has largely revolutionized the job market and has somewhat leveled the playing field. If you choose an unconventional path (say, an artist), then be prepared for your income to be middling at best or be beset with financial problems at worst. To be a lawyer, doctor, government employee, engineer or entrepreneur means a sure path to financial freedom. So, what is Jumpcut Academy and what can it do for you?īefore the arrival of the internet, only people with conventional or prestigious jobs make enough money to finance their lifestyle. ![]() If you've ever aspired to become an Instagram influencer or Youtube superstar who rakes in cash every day, then you'd probably be interested in Jumpcut Academy. ![]() You won’t see fantails swimming freely in the wild like their ancestors because they are a specially bred bunch. They originated in China and Japan, but you can easily find them almost anywhere in the world today! Being a species of goldfish, the fantail goldfish ( Carassius auratus auratus) share the same history. Skilled Asian breeders meticulously bred the fancy goldfish that we see today to bring out their mesmerizing colors and beautiful shapes. Over the years, they’ve spread to Japan, Europe, and later to America. Unlike their wild ancestors, goldfish are a result of centuries-long selective breeding, originating in Ancient China about 1,000 years ago. In fact, they are pretty common in western countries. They are direct descendants of Carassius gibelio that also includes wild Prussian carp.Īlthough Native to East Asia, they are found all over the world. Let’s turn a few pages of history and learn about the origins of goldfish, shall we? The goldfish is a small but prominent member of the carp family, basking in the glory of being considered one of the finest aquarium fish for centuries. 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Basemark GPU is a benchmark software for Linux and other operating systems to measure the performance of the graphical processing units installed on your system. ![]() Patrick’s Day Parade Committee, the Women of Irish Heritage, and the Ancient Order of Hibernians Div. Food Drive for West Orange – In coordination with the West Orange St.Luck O’ the Pup – Pet adoption event and fundraiser hosted in support of several local animal rescue shelters.Baldrick’s Foundation for childhood cancer research Baldrick’s Foundation – Fundraising event in support of St. Brendan Tevlin Lacrosse Festival – Raises funds for charitable endeavors and promotes “Good Vibes & Easy Living”.Back to School Yard Party – Aims to supply a fully-stocked backpack to students in need from Thomas A.Irish Sports Expo – A celebration of Irish sport and culture welcoming in everyone from the local community to experience hurling, rugby, and Gaelic football, as well as Irish music and dance.Support the Troops Care Package Drive – Drive to collect and purchase materials to fill care packages for US servicemen and women serving our country throughout the world.Scholarships – In coordination with the Bannon Scholarship Foundation, provides five scholarships totaling $5,500 to support local high school and college/post college students.Annual Essex Shillelagh Dinner Dance – To honor brother members and friends of the Shillelagh Club for their outstanding service.Patrick’s Day Parade and the parade committee, who put on one of the best St. Patrick’s Day Parade – For as long as we have been in West Orange, the Friendly Sons have been ardent supporters of the West Orange St. Irish Cocktail Party – For members of our local Irish heritage organizations.Below is a brief description of some of our hallmark annual undertakings: Detailed information about each event can be found in the “Events” section above. Throughout the year, we host and/or support multiple charitable endeavors, cultural events, and community outreach functions. Our Foundation is a registered 501(c)(3) charitable organization, continually working to help our community. ![]() We are an Irish-American cultural and social organization who work to preserve our Irish traditions and heritage and to share them with the community at large. Patrick’s Day Parade, Tom Murphy and Bill Byrne were the Drum Majors.The Order of the Friendly Sons of the Shillelagh–Essex Division, has been a pillar of the West Orange community for over 50 years. ![]() In 19 the Band had the honor of leading the Newark St. ![]() In 1976, the Band was awarded Best Foreign Group Trophy, Jerry DeYoung was the Drum Major. In 1970, the Band was awarded the Mayor’s Trophy, Jerry Kennedy was the Drum Major. The band has completed and won recognition in numerous Pipe Band Competitions and has over the years participated in hundreds of parades, exhibitions and shows. * Former Pipe Majors still active in the band. The following have served with distinction as Pipe Major: This band was instructed by George Bell, Piping, and James Cairns, Drumming. The original Pipe Band/Club Members that marched This initiative, taken concurrently with building our club house, demonstrated the fine quality of leadership that our club history depicts. The large financial burden of establishing the band, purchasing pipes, drums, and uniforms, and paying for piping and drumming instruction was borne by The Club. Credit for supporting the idea of a club Pipe Band and the driving force behind establishing it was our club president, Jack Dunphy. was the first parade for our Pipe Band, fully outfitted in new uniforms. What typically happens on Reddit is that the more active you are, the more people become interested in you as a person. This is the easiest and most natural way to build relationships with members of the community. There are tons of rappers, producers, engineers, and other hip-hop oriented people in the MakingHipHop subreddit for you to connect with. Next, you should join an active subreddit like /r/MakingHipHop and start contributing to the discussion. “Self-post” are usually looked at as non-spammy and makes it more likely for people to take interest if they stumbled upon subreddit. If you’re unfamiliar with Reddit, a “self-post” is simply a post that contains text instead of redirecting to an external website. I’d recommend making them a “self-post” in the beginning. The main goal of this is to form a community for you to post your music in. For example, you can name it “Cleveland Rappers” or “*Insert City* Rappers”. You can name your subreddit after your rap name, group name, or you could go broader. Then the second thing you should do is start your own subreddit. This process is short and won’t take more than a couple of minutes. You can't go in there and say, "Hey, check out my music." – It doesn't work like that.īefore doing anything, you need to create an account Reddit. Reddit is not a place for spam or BS because they spot spam from a mile away. ![]() This guide is tailored towards hip-hop artist, but can be used for any genre.Before I give you a method for promoting your music on Reddit, I want to tell you that: Some concept art depicted her with a group of pet ermines who scurried up and down her body and formed themselves into a cloak for her. These designs depicted Elsa with spiky black or dark blue hair (and even blue-gray skin), a more angular body (as opposed to the final, heroic Elsa's curvaceous body) and a constantly malicious smile. Designs for this version of Elsa resembled such figures as actress Bette Midler, and the late singer Amy Winehouse. Early concept art and visual development depicted the character-eventually named Elsa-as being a ruthless, bitter, cold-hearted tyrant who had an entire army of giant snowmen as henchmen. Ī common consensus was that Disney's iteration of the Snow Queen would be portrayed as a villain. ![]() One of these attempts almost went as far as to pair an aged-up Kai with the Snow Queen while vilifying Gerda. A common theme in adaptation attempts in the late 1990s and early 2000s was to reconfigure the story into a romantic comedy about the Snow Queen's ice-cold heart-melting as she learns to love. In the tale, the Snow Queen resides in an ice palace coated in permafrost and guarded by such animals as polar bears and porcupines.ĭisney's decades long efforts to adapt The Snow Queen generally shared a common thread of trying to find a way to expand on the role of the title character and make her a more active presence in the story. Kai's disappearance leads his childhood friend, Gerda, to embark on a quest to find him. The Queen promises to break the mirror's spell if Kai can spell “eternity” with pieces of ice in her palace. In Hans Christian Andersen's original story, the Snow Queen abducts a young boy named Kai, who had fallen victim to the machinations of an evil mirror. It is believed by historians that The Snow Queen was intended to be one of the project's animated sequences, but there is no evidence of creative work for the proposed feature. There were talks regarding a live-action biography on Hans Christian Andersen, which would have featured animated segments based on his works. Walt Disney's adaptation of The Snow Queen was given a production number in 1939. Davis was enraptured by the tales of Hans Christian Andersen, and proposed they be adapted to animated shorts at the studio. ![]() Marc Davis, one of Disney's key animators, traveled to Scandinavia on Walt's orders to research the region's books and stories. DevelopmentĪn early concept of the Snow Queen by Harald Siepermann.Īfter the success of Snow White and the Seven Dwarfs in 1938, Walt Disney sought out new fairytales to serve as the basis for future productions. She fears she's becoming a monster and that no one, not even her sister, can help her. Her mounting emotions trigger the magic, accidentally setting off an eternal winter that she can't stop. Haunted by the moment her magic nearly killed her younger sister Anna, Elsa has isolated herself, spending every waking minute trying to suppress her growing powers. It's a beautiful ability, but also extremely dangerous. 4.3 Conceal, Don't Feel (A Twisted Tale)īackground Official Description From the outside, Elsa looks poised, regal, and reserved, but in reality, she lives in fear as she wrestles with a mighty secret - she was born with the power to create ice and snow.4.1 Anna & Elsa: Sisterhood is the Strongest Magic.The Disney adaptation expanded the Snow Queen to serve as a villain initially, but the advent of " Let It Go" inspired the filmmakers to rewrite the character as a tragic heroine. Though pivotal to its events, the character had little presence in the original story. She thereby abdicates the throne, allowing Anna to rise as the new queen of Arendelle.Įlsa is loosely based on the titular character of " The Snow Queen", a fairy tale by Hans Christian Andersen. Three years into her reign, Elsa is called forth to Ahtohallan to assume her rightful place as the Fifth Spirit of the Enchanted Forest, whose purpose is to bridge the magic of nature and people. Through Anna's love, however, Elsa was able to control her powers and live peacefully amongst her people with a newfound self-confidence. Elsa's anxieties would eventually trigger a curse that plunged Arendelle into an eternal winter. Therefore, she isolated herself from the world as a means of protecting her family and kingdom. ![]() Throughout most of her young life, Elsa feared that her powers were monstrous. Born with the power of ice and snow, Elsa is the firstborn daughter of King Agnarr and Queen Iduna, the older sister of Queen Anna, and the former queen of Arendelle. ![]() “ In Arendelle's fair kingdom, a ruler did appearīorn with a secret power so great, alone, she stayed in fearĪlthough the force was hidden, one day she let it goĪnd all the land was covered in eternal ice and snow” ― Frozen trailer's narrationĮlsa the Snow Queen is the deuteragonist of Disney's 2013 animated feature film, Frozen, and the protagonist of its 2019 sequel. Continued abuse of our services will cause your IP address to be blocked indefinitely. ![]() Please fill out the CAPTCHA below and then click the button to indicate that you agree to these terms. If you wish to be unblocked, you must agree that you will take immediate steps to rectify this issue. 2nd Dec 2006 (JPN) 12th Jan 2007 (UK/EU) 15th Jan 2007 (NA) 15th Sep 2016 (NA) 6th Oct 2016 (NA) 007: Quantum of Solace Wii. 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